Histologic preparation of the sections includes staining using the Villanueva Osteochrome Bone Stain, undecalcified solution processing, and embedding in polymethylmethacrylate. The embedded specimens are cut at a thickness of 250µm to 300µm, then ground and polished to 120µm. High resolution microradiographs are then obtained at this point.

The resulting histologic slides depict a highly readable and accurate assessment for histomorphometric quantification of trabecular bone area at an implant interface, wear debris, and signs of inflammatory giant cell reactions, degenerative changes or autolysis.